Preliminary Program – BioParadigms 2001

Program – Biomedical Transporters 2001

Interlaken, Switzerland

Sunday, August 5

4-8 PM Registration

6-10 PM Reception

Monday, August 6

Welcome

Matthias A. Hediger (Harvard Medical School, Boston, MA), Robert Burrier (GelTex Pharmaceuticals, Inc., Waltham, MA), Wolf Frommer (University of Tübingen), Peter Meier-Abt (University of Zürich, Switzerland), Heini Murer (University of Zürich, Switzerland)

Overview - Transporter Superfamilies: Matthias A. Hediger

Session I – Mono- and disaccharide transporters: Chairs Wolf Frommer and Ernest Wright

Ernest Wright (University of California, Los Angeles) - The SGLT Family (SLC5): Structure and Diverse Function of Sodium Cotransporters

Mike Mueckler ( Washington University School of Medicine, St. Louis, MO) – The SLC2 family of glucose transporters (GLUT1 to GLUT8)

Eckhard Boles ( University of Düsseldorf, Germany) - Glucose transporters as targets for drug screening: using yeast mutants as a heterologous screening system

Widmar Tanner (University of Regensburg, Germany) - Glucose transport in plants

Session I – Continued

Wolf Frommer (University of Tübingen) - The disaccharide transporter superfamily: Sucrose transporters in plants

Session II – The oligopeptide transporter superfamily (SLC 15): Chair Ron Barrett

Hannelore Daniel (Technical University of Munich) - The peptide transporter superfamily: Physiological and pharmacological implications

Ron Barrett (Xenoport, Inc., Palo Alto, CA) – Improving Drug Bioavailability by Engineering Active Transport

Session III – Amino acid transporter superfamilies: Chair Manuel Palacin

Manuel Palacin (University of Barcelona, Spain) – The Amino Acid Permease Superfamily (SLC7) and cystinuria

Sela Mager (University of North Carolina, Chapel Hill, NC) – The GABA/neurotransmitter/amino acid transporter superfamily (SLC6)

Michael Kavanaugh (Oregon Health Sciences University, Portland, OR) - Glutamate transporters: Structure, pharmacology and roles in physiology and pathophysiology (SLC1 superfamily)

Jeffrey Erickson (Louisiana State University, New Orleans, LA) – The SLC38 (Systems A and N) and SLC17 (vesicular glutamate transporters) superfamilies

Evening - Enjoy Interlaken! (No planned events)

Tuesday, August 7

Session IV – The nucleoside, bile acid, organic anion, organic and cation transporter superfamilies (SLC10, 21, 22, 28 and 29) - Chair: Kathleen Giacomini

Kathleen Giacomini (University of California San Francisco) – Organic cation and nucleoside transporters, and their relevance to drug absorption and elimination

Peter Meier (University of Zürich, Switzerland) - Bile acid and organic anion transporters

Hitochi Endou (Kyorin University, Tokyo) - Role of SLC22 family members in human pharmacokinetics

Jungfraujoch Excursion

Evening

Session V – Novel insights into the structure and function of transporters and channels: Implications for drug discovery (Chair Alan Verkman, University of San Francisco)

Joe Mindell (Brandeis University, Waltham, MA) – Crystal structure of a ClC channel

Alan Verkman (University of California San Francisco) - CFTR and aquaporins as targets for drug discovery

Yoshikatsu Kanai (Kyorin University School of Medicine, Tokyo) – System T amino acid transporter: Molecular identification and pharmacological properties

Vladivel Ganapathy (Medical College of Georgia Augusta, GA 30912, USA) – Pharmacology and therapeutic use of the amino acid transporter ATB0,+

Wednesday, August 8

Session VI – Drug discovery strategies and technologies (Chair Robert Burrier, GelTex Pharmaceuticals, Inc., Waltham, MA)

Robert Burrier(GelTex, MA) - Introduction on the Integration of Drug Discovery, High-throughput Screening, and Transporter Biology

Werner Kramer (Aventis Pharma, Frankfurt, Germany) - Discovery of Inhibitors of the Sodium Dependent Bile Acid Transporter

Thomas Large ( Eli Lilly & Co., Indianapolis, IN) - Choosing a high throughput screening format for ion channels and transporters.

Michael Xie ( Millennium Pharmaceuticals, Cambridge, MA) - Assay development for electrogenic transporters using a novel fluorescent membrane potential dyes

Tito Gonzalez (Aurora Biosciences, San Diego, CA) - High-throughput technologies for ion transport functional analysis and drug discovery

Lisa Higgins (Elan Corporation, Dublin) - The use of TOGA(TM) to identify membrane receptors and transporters in in
vitro and in vivo models of human Peyer's patch M cells

Session VII – Physiological Implications and commercial opportunities in the transporter field (Chair George Sachs, University of California Los Angeles)

George Sachs – UreI-mediated urea transport in Helicobacter pylori

Heini Murer (University of Zürich, Switzerland) - Epithelial phosphate transporters

Anne-Marie Marini ( Universite Libre de Bruxelles, Belgium) - From yeast ammonium transporters to human Rhesus polypeptides

Donald Hilgemann (University of Texas Southwestern) - Lipid signaling and ion transport

Soraya Shirazi-Beechey (University of Liverpool, UK) – Molecular characterisation and regulation of a monocarboxylate transporter (MCT, SLC16) in human colon

Stefan Silbernagl (Universität Würzburg, Germany) – Renal Tubular Transport of myo-inositol in vivo

Evening

Special Dinner/ Entertainment (Swiss Evening)

Thursday, August 9

Session VIII - Fatty acid and cholesterol transporters (SLC27, ABC transporters, etc.) (Chair Louis Tartaglia, Millennium Pharmaceuticals, Cambridge, MA)

Richard M. Lawn (CV Therapeutics, Palo Alto, CA) - ABC transporters and cholesterol metabolism

Monty Krieger (Massachusetts Institute of Technology, Cambridge, MA) - Charting the Fate of the "Good Cholesterol" - Physiologic and Genetic Analysis of the HDL Receptor SR-BI

Nada Abumrad (Stony Brook, NY) - The role of CD36 in facilitating fatty acid transport

Ruth Gimeno (Massachusetts Institute of Technology, Cambridge, MA) - Fatty Acid Transport Proteins (FATPs)

Session IX – Membrane transporters in the choroid plexus (Chair Jean-François Ghersi-Egea, INSERM, Lyon, France)

Jean-François Ghersi-Egea - The blood-brain interfaces in cerebral pharmaco-toxicology. Contribution of an in vitro cellular model to study choroidal transport

Michel Lemaire (Novartis Pharma AG, Basel, Switzerland) - Importance of blood-brain interface transporters in drug development

Kathleen Giacomini (University of California San Francisco) – Nucleoside transporters at the CP (tentative)

John B. Pritchard (NIH, Research Triangle Park, NC) - Organic anion transporters (OAT) and organic cation transporters (OCT) families at the choroid plexus

Yuichi Sugiyama (University of Tokyo) - Drug transport and efflux at the choroid plexus: from in vivo studies to molecular identification of transporters

Choroid plexus/hepatobiliary Keynote Lecture

Peter Meier (University of Zürich, Switzerland) - "Epithelial Transport Polarity:

Similarities and differences between the choroid plexus and the liver"

Evening

Choroid Plexus/Hepatobiliary Dinner

Session X - Hepatobiliary transport session (Chair - Bruno Stieger, University of Zürich, Switzerland) (7:30 pm to 9:30 pm)

Deitrich Keppler and Jörg König (Deutsches Krebsforschungszentrum, Heldelberg, Germany) - ABC-transporters in the liver

Michael Muller (Wageningen, The Netherlands) - Transcriptional regulation of hepatocellular transporters

R. Oude Elferink (Amsterdam, The Netherlands) - Hepatic cholesterol and lipid transport

Friday (August 10)

Hepatobiliary Transport Satellite Meeting

Organizers: Bruno Stieger (University of Zürich), Peter Meier-Abt (University of Zürich), Matthias A. Hediger (Harvard Medical School) and Oude Elferink (University of Amsterdam)

7:30 - 8:00 AM Registration

8:15 AM
Opening Remarks: Peter Meier and Bruno Stieger (University of Zürich, Switzerland)

Session H1: Gerd A. Kullak-Ublick (University Hospital Zürich, Switzerland) - Chair

8:30 AM:
Jenny Ros and Klaas Nic Faber (University Hospital Groningen, The Netherlands) - ABC Transporter Gene Expression in Hepatic Progenitor Cells

9:00 AM
Diana Jung and Gerd A. Kullak-Ublick (University Hospital Zürich, Switzerland) - Regulation of Liver-Specific OATP-Genes by Hepatocyte Nuclear Factor 1

9:30 AM
Michael Trauner and Gernot Zollner (Karl Franzens University, Graz, Austria) -
Role of the Transcriptional Regulation Repressor SHP-1 for Down-Regulation of Ntcp in Bile Duct Ligated Mice

10:00 - 10:30 AM: Coffee Break

Session H2: Dirk Meijer (Groningen University Institute for Drug Exploration, The Netherlands) - Chair

10:30 AM
Marieke Eleferink and Dirk Meijer (Groningen University Institute for Drug Exploration, The Netherlands) - Regulation and Perturbation of Hepatobiliary Membrane Transport in Liver Disease

11:00 AM
Ulrich Beuers and Christian Rust (Klinikum Grosshadern, University of Munich, Germany) -Taurolithocholic Acid Impairs Hepatobiliary Exocytosis, Bile Acid and Organic Anion Secretion by a Phosphadiylinositol 3-Kinase-Dependent Mechanism

11:30 AM
Christoph Dietrich and Ronald Oude Elferink (Academic Medical Center, University of Amsterdam, The Netherlands) - Mrp2-Mediated Liver Injury, the Flip-Side of Concentrative Transport

12:00 Noon
Carlo Spirli (Department of Medical and Surgical Sciences, University of Padova, Italy) - Glibenclamide-Stimulated Secretion in Bile Duct Epithelium and Role of p-gp

1230-1330: Lunch

Session H3: Ronald Oude Elferink (Academic Medical Center, University of Amsterdam, The Netherlands) - Chair

1:30 PM
Edith Friesema and Theo Visser (Erasmus University Medical School, Rotterdam, The Netherlands) - Characterization of Hepatic Thyroid Hormone Transporters

2:00 PM
Jane Bryne, Sandra Strautnieks and Richard Thompson (King's College Hospital London, UK) - University Hospital Groningen, Groningen, The Netherlands) - The Problems of Expressing Human BSEP in vitro

2:30 PM
Anne Figge and Frank Lammert (Universitätsklinikum, Rheinisch-Westfalische Technische Hochschule, Aachen, Germany) - Genomic Analysis of Hepatobiliary Lipid Transport in Inbred Mouse Models

3:00 PM
Saskia van Mil and Leo Klomp (University Medical Center Utrecht, Utrecht, The Netherlands) - Mechanisms of Copper Homeostasis: Lessions from Yeast and Man

3:30 PM

Peter and Meier and Bruno Stieger - General Discussion: Future of the European Heptobiliary Transport Group